Soil seed bank of weed

A Comparative Study of Soil Weed Seed Bank Determination in Pothwar Region by using Different Methodologies

1 Department of Agronomy, Pir Mehr Ali Shah, Arid Agriculture University, Rawalpindi, Pakistan; 2 Ecotoxicology Research Institute, National Agricultural Research Centre Islamabad, Pakistan; 3 Crop Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan; 4 Research Farm Crops, Agriculture Department, AJ and K, Baldmas District Kotli, Pakistan.

Abstract | Soil weed seed bank is a natural source for weed infestation. Determination of soil weed seed bank has primary importance to get complete picture of weed seed reservoir in the soil profile. An experiment was conducted at University Research Farm, Chakwal Road, Rawalpindi to compare the techniques for determination of soil weed seed bank during winter 2012-2013 under rainfed conditions of Pothowar. Soil samples for the weed seed bank analysis were taken from the experimental field before wheat sowing from 0-10 cm, 11-20 cm and 21-30 cm soil depth. Two soil weed seed bank determination techniques were compared viz., sieving method and seedling emergence method. The data were collected on the seed density m -2 , seed frequency, diversity of weed species and relative importance value. The input and output data was also collected to find out the socioeconomic feasibility of the techniques. The comparative analysis of seed bank extraction methods revealed higher weed seeds density, weed frequency with more diversity of weed species under sieving method in comparison to seedling emergence method. Therefore, sieving method was considered superior over seedling emergence method. The feasibility analysis of seed bank extraction methods indicated that sieving was cost-effective, less time consuming, more user friendly with higher accuracy over seedling emergence method.

Received | September 29, 2017 ; Accepted | October 27, 2017 ; Published | November 21, 2017

*Correspondence | Ijaz Ahmad, Ecotoxicology Research Institute, National Agricultural Research Centre Islamabad, Pakistan; Email: [email protected]

Citation | Hussain, M., S. Ali, M.N. Tahir, G.A. Shah, I. Ahmad, M.A. Sarwar and S. Latif. 2017. A comparative study of soil weed seed bank determination in pothwar region by using different methodologies . Pakistan Journal of Agricultural Research , 30(4): 310-315.

Keywords | Comparative study, Weed seed bank, Sieving method, Seedling emergence method

S oil acts as storage house for different macro and microorganisms including insects, micro-organism, fungi, algae, spores, nematodes and seeds of different weeds. Weed plants after maturation shed their seeds and these weed seeds ultimately accumulated in the soil profile which form weed seed bank in the soil. Soil weed seed bank comprises of all viable, dormant and non-dormant seeds present in the soil profile ( Forcella et al., 2003 ). Knowledge of soil weed seed bank is important for population dynamics studied, establishment of appropriate weed management programs ( Ambrosio et al., 2004 ) and forecasting of weed infestations ( Ball and Miller, 1989 ; Creech et al., 2008 ).

Weed seeds may enter in the seed bank through many sources from plant seed production, together with primary and secondary dispersal such as farm equipment, contaminated crop seeds, animals, wind and manure ( Buhler et al., 1997 ). Among these sources, the largest source of weed seeds in the seed bank is plants producing seed within the field. Weed seeds also drive the spread of weed patches in fields, both for annual ( Steinmann and Klingebiel, 2004 ) and perennial weed species ( Blumenthal and Jordan, 2001 ), and are the only source of population increase for annual weed species. Decline in weed seed bank may occur by various factors such as germination, seed predation ( Van Mourik et al., 2005 ), seed decay and death ( Gallandt, 2006 ) and deep seed burial to layers from where emergence onto the soil surface is impossible ( Honda, 2008 ). These seed banks range from near 0 to as much as 1 million seeds m -2 ( Radosevich et al., 1997 ).

There are a number of methods that have been used to determine the density and composition of soil weed seed bank. These methods are categorized into two main techniques that are used to find out the number of seeds from the soil samples, i.e. (1) weed seed extraction method and (2) weed seedling emergence method. In direct seed extraction method, weed seeds are extracted by washing and floatation methods while in the second technique, weed seedling emergence, the soil sample is placed in the greenhouse or controlled environment, watered on regular basis in order to emerge the weed seedling and these emerged seedlings are then identified and counted ( Luschei, 2003 ).

The objective of current study was the determination of soil weed seed bank of Pothowar region and to compare different methods to find out the most accurate, efficient, handy and economical technique for the determination of soil weed seed bank.

Material and Methods

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A comparative study of soil weed seed bank determination methods was conducted during 2012-2013. Soil sampling was done from the different localities of the wheat field at University Research Farm, Chakwal Road Rawalpindi. Sampling of the soil was carried out before the sowing of wheat crop diagonally in four replications from three depths i.e. 0-10 cm, 11-20 cm and 21-30 cm. Soil samples were taken by using steel probe of 2.5 cm diameter for each method from each replication. The soil cores of same depth were bulked and mixed to make composite soil samples. These composite soil samples were divided into three working sub-samples having one hundred gram weight of each sample for soil weed seed bank analysis. The soil samples were then transported to laboratory and stored at room temperature until further processing. Two soil weed seed bank determination techniques i.e. seed extraction using sieving method and seedling emergence method were compared for extraction of weed seeds from soil. In sieving method, seeds were extracted from soil by sieving of soil sample through various sieves with different mesh sizes using method adopted by Konstantinović et al. (2011) . Each 100 gram soil sample was initially poured on sieve of 80 mesh size and placed in the water for softening the soil clods. This sample was then immersed in the sodium hexa-metaphosphate solution (40 g/L of water) in order to disintegrate the soil particles. The soil samples were shifted to the bucket having tap water and shook well so that almost all clay and silt particles were filtered out and removed from sample. The remaining material on the sieves was air dried and transferred on the filter paper so that samples become completely dried. These dried samples were then passed through a descending series of sieves i.e. mesh no. 10, 18, 30, 40, 50 and 80. Entire seeds remained on the sieves were collected for identification and further processing. Seeds of different weeds were collected from experimental area and its surrounding with objective of reference collection for the weed seeds identification. Seeds extracted from soil were compared with the collected seeds to identify seeds using high magnification lens (10X) and seeds of each species were counted. Viability of seeds was determined by using crushing method, i.e. gentle pressure was applied to the seeds with the help of forceps and seeds that resisted this pressure were considered as viable and counted. Seedling emergence trial was carried out in the growth chamber keeping the controlled environment for germination of different Rabi weed seeds. Soil core samples weighing 100 gram were spread onto petri dishes in separate sections according to their depth and replication. These petri dishes were placed in the germination chamber. The temperature in the germination chamber was maintained from 20 ˚C during day time (12 hours) to 8 ˚C during night time (12 hours). Petri dishes were watered on daily basis avoiding samples to dry. Emergence of weed seedlings was observed on weekly basis. Emerged weed seedlings were identified

Harvest Weed Seed Control – How It Depletes Soil Seedbank

Some Australian farmers collect chaff in pull-behind carts like this one to keep weed seeds from dispersing.

Some Australian farmers collect chaff in pull-behind carts like this one to keep weed seeds from dispersing.

The Soil Seedbank

“Soil seedbank” refers to weed seeds present in the soil. The soil seedbank serves as the source of next season weed problems. The majority of seeds in the soil were deposited by plants that escaped control on the last year. Due to seed dormancy, weed seeds can remain viable in the soil for many years and often emerge over many weeks during the growing season.

Seed dormancy, prolonged viability, and extended emergence periods make it easier for weeds to survive across a wide range of environments and management tactics. Current management recommendations target weed seed production to prevent additions to the soil seedbank. Recommendations include controlling weeds while they are small and susceptible to weed control tactics, removing weeds that escaped control, and managing weeds that produced viable seeds to minimize their impact on the soil seedbank.

These late-season practices are not new, but due to wide-spread herbicide-resistance they are being revisited with new technology. In this video Dr. Mandy Bish explains the importance of understanding the weed seedbank and why preventing weeds from producing seeds is so important.

How to Manage the Soil Seedbank?

Currently there are no economical methods to kill weed seeds once they enter the soil. Weed management practices focus on controlling weeds early in the growing season with herbicides and cultivation, then following up to manage those weeds that escape control and prevent seeds from entering the soil.

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Studies have demonstrated that preventing seeds from returning to the soil can rapidly reduce the seedbank and resulting weed densities. Allowing weeds to produce viable seeds, however, can cause rapid increases in weed densities. Stopping seeds from entering the seedbank can be achieved by

  1. Preventing weeds from producing seeds (no seeds, no weeds)
  2. Enhancing weed seed predation
  3. Increasing seed mortality
  4. Removing seeds from the field before the seeds are shed
  5. If weeds do produce viable seeds, place seeds in narrow strips with chaff lining to improve management next year.

There has been a renewed emphasis on increasing seed mortality, mechanically removing seeds from the field, and altering the placement of seeds. These were tactics used before the wide-spread use of herbicides. With the technological advances in machinery these tactics are again being researched and adapted for use in various regions.

Palmer amaranth that escaped control during the growing season, with viable seeds ready to return to the soil seedbank. Photo: Claudio Rubione

How to Prevent Adding to the Soil Weed Seed Bank

As cash crop harvest approaches you may discover that weeds have escaped your control efforts and are setting seeds. These weeds may be very visible, poking their heads above the canopy of the cash crop. When you go to harvest the cash crop, you are at risk of spreading the weed seeds.

These escaped weeds will create an even bigger problem next year if they are allowed to add the seeds they have produced back to the soil weed seed bank, or to spread their seeds to new fields by hitching a ride on harvesting equipment. You still have a chance to prevent this from happening.

Scout your fields prior to harvest.

You can scout using drones or other means such as physically walking the fields.

Know where the problem areas are: map them out.

Plan to harvest weed-free areas first and weedy areas last.

Limit the spread of weed seeds through the field and from weedy fields to clean ones. Clean the combine before leaving a field. A single Palmer amaranth plant can produce hundreds of thousands of seeds – planning your harvest strategy and cleaning equipment diligently is well worth the time. Remember: scout, map, and plan prior to harvesting to Get Rid of Weeds!

When to manage the soil seedbank?

Prior to planting

The most common tactics to reduce the soil seedbank include crop rotation and tillage. Diverse crop rotations allow for a diversification of other supporting IWM tools, such as herbicides, tillage, and crop competition. Diversified crop rotations are rarely used to manage the soil weed seedbank. The use of only one (or a few) cash crops ultimately allows weeds with similar traits to thrive, replenishing the weed seedbank annually. Tillage can both increase the soil seedbank (by burying seeds) and decrease it (by unearthing buried seeds that may germinate early and die).

The effect of deep tillage on the soil seedbank can be gauged by knowing the main weed species in a field and understanding how long the seeds of the species stay viable: deep tillage is a better choice for short-lived seeds. Grass weeds tend to have less persistent seeds than broadleaf weeds. Strategic deep tillage affects seedbanks depending on weed traits such as germination and seed size.

A specific tillage tactic to manage the seedbank is the stale seedbed method. Stale seedbed management uses tillage to promote weed germination so that another management tactic can then be used to kill weed seedlings prior to crop planting, reducing the size of the soil seedbank. Crop rotations and tillage are fundamental for weed management, but management decisions tend to prioritize economic and logistic factors over effects on weeds.

Prior and or during harvest

Harvest Weed Seed Control (HWSC) is an approach to managing the soil seedbank. These methods focus on cultural and mechanical options to reduce the impact of seeds from escaped weeds at harvest time. These are only effective for weed species that do not shatter and allow their seeds to shed prior to mechanical harvest.

HWSC tactics include:

  1. Narrow windrow burning
  2. Chaff lining
  3. Chaff tramlines
  4. Impact mills
  5. Chaff carts
  6. Bale direct system

Narrow windrow burning is relatively easy to adopt and can provide good results. Both small and large size weed seeds are controlled. This tactic involves a chute that is attached to the rear of the combine that concentrates the chaff and straw residues into a narrow windrow, which is later burned. At around $250 in materials it is also cost effective.

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However, this method is time consuming, removes most of the field ground cover, has a risk of fire escape, may not be legal in all jurisdictions, is not an option for all crops (e.g. corn), and it is difficult to achieve a good burn in long windrows. Narrow windrow burning requires some trial and error. Harvesting low is key to obtaining more crop residue to burn.

Choosing the right time and conditions to burn is critical. A light wind is ideal, but be careful of a gusty day. Current research suggests that narrow windrow burning works best in wheat and soybean cropping systems, but other systems are being tested.

Windrow formation for burning in a wheat field highly infested with Italian ryegrass in Arkansas. Photo: Lauren Lazaro Windrow burning needs to be carefully planned ahead of time. Wind speed and direction, relative humidity, availability of tillage equipment to build preventive fire fences, and access to plenty of fire extinguishers are some of the issues to be considered for safely managing the fire. Photo: Claudio Rubione

Chaff lining is a cost effective HWSC method. Chaff lining takes a chute and diverts only the chaff fraction into a narrow row in the center of the harvester, while the rest of the crop residue is spreadly evenly behind the combine. While weed seeds are returned to the soil, they are in narrow lines instead of being spread across the entire field.

The chaff material is allowed to rot and decay. These lines could be treated differently, using targeted herbicides sprays, or managed with different tools at a site specific level.

A plastic chute fitted to the harvester funnels the chaff containing the majority of weed seeds present into a narrow band in the middle of the CTF run. Photo: Mic Fels

Chaff tramlining forms the chaff material into narrow rows on dedicated wheel tracks during harvest and relies on a mulch effect to prevent weed seed germination and emergence, as in chaff lining (above). Chaff tramlining equipment runs around $15,000 to $18,000 depending on your harvester brand and model.

Chaff tramliners are variable and quite expensive. Photo: Peter Newman, WeedSmart

Impact mills, such as the integrated Harrington Seed Destructor or the Seed Terminator, are integrated into the rear of the combine where an impact mill physically destroys the weed seeds in the chaff fraction.

Weed seed contained in the chaff fraction will depend on the weed species and in how many of the seeds are retained on the weed before harvest Impact mills can be highly effective with over 95% destruction of the weed seeds that enter the mill. The current impact mills are expensive and not available in most countries.

Integrated Harrington Seed Destructor. Collected chaff is sent to the mills, which pulverize weed seeds and small residue into dust, which comes out through the back of the machine. A four year study on the technology is being carried out across the US. Photo: DeBruin Brothers

Chaff carts are the simplest HWSC system, consisting of a chaff collection and transfer mechanism that is attached to a combine that delivers the chaff fraction to a bulk collection bin, usually a trailing cart, that can be physically removed from the field so the chaff can be burned or grazed.

Major downsides to this method are that it adds extra length to the combine so it can be difficult for small fields or fields without much room to navigate and it requires extra time to empty the carts.

Chaff cart pulled behind a combine in Australia. Photo: Michael Walsh, Australian Herbicide Resistance Initiative

The bale direct system consists of a large square baler that is attached directly to the harvester which constructs bales from the chaff and straw residue. There is a limited market for the bales and a large risk of immediately spreading weed seeds to other fields.

The bale direct system provides a new system for harvesting corn and baling corn stover in one simple step. Picture credit:

All of the HWSC methods are similarly effective, but come with different initial costs, operating costs, and residual costs. Additional pros and cons related to feasibility, and level of nutrient removal or redistribution should be considered. The best HWSC method for your farm comes down to cost and other management concerns.

Table 1: Pros and cons of the six different HWSC methods.

High capital cost

While the cost of managing the soil seedbank ranges dramatically by tactic, the end goal is ultimately the same. In the future, knowledge about the links between seedbank density and weed emergence patterns may inform additional soil seedbank management practices.